How Does Copper Silver Ionization Penetrate Biofilm? Biofilm is normally made from organic living matter, organic dead matter and inorganic materials. Biofilm is known to build-up over time inside a facility’s potable water distribution network where it can protect and nourishes Legionella bacteria. This in turn can provide ideal growth environments that eventually become problematic for Legionella growth & exposure. CSIDefender® releases positively charged copper and silver ions which saturates this biofilm in 30 – 45 days, creating an environment where Legionella can no longer freely grow and can prevent future growth of this bacteria.